PCR重组体是什么？‘PCR recombinants’ (also known as PCR chimeras？

发布网友发布时间：2022-05-16 06:17

共1个回答

热心网友时间：2023-10-13 10:19

在PCR时，模板发生漂移，最多见于16sRNA。

A chimera is a spurious gene sequence derived from more than one DNA template (Meyerhans et al., 1990; Shuldiner et al., 1989). Chimeras form ring PCR amplification, where sequence synthesis starts from one template, is interupted (for example, through damage or degradation of the template; Pääbo et al., 1990), and then continues from another template sharing a degree of localised homology to the original. In this way, a chimera can form from two or more phylogenetically distinct gene fragments, derived from different parental templates. Clearly, chimera formation has the potential to occur when there is more than one DNA template available within the PCR mixture; as is the case when PCR amplification is performed on pure DNA samples contaminated with foreign genetic material, or, as is increasingly common, on DNA extracted from environmental or medical samples (e.g., Kopczynski et al., 1994).

http://www.bioinformatics-toolkit.org/Help/Topics/chimera.html