什么是OT-II和OT-I小鼠?
发布网友
发布时间：2022-05-23 06:50
共2个回答
热心网友
时间：2023-09-20 09:50
OT-II小鼠是体内CD4+T细胞特异性识别OVA的小鼠，OVA是一种卵清蛋白。
OT-I小鼠是体内CD8+T细胞特异性识别OVA的小鼠。
这两种小鼠主要用于研究肿瘤免疫治疗，通过注射表达OVA的肿瘤细胞，再给予小鼠相应的T细胞，可以评估其对肿瘤的特异性免疫反应。
热心网友
时间：2023-09-20 09:51
These transgenic mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b. Homozygous mice are viable and fertile. In these mice there is a four-fold increase in the CD4 to CD8 peripheral T cell ratio, and lymph node T cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand. These transgenic mice are useful for studying in vivo T cell biology such as TCR-ligand interactions, T cell activation, thymic selection, cross-presentation of antigens, process of thymic selection and central and peripheral T cell tolerance and inction.
OT-I: (CD8+---------ovalbumin 257-264 )
These mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was designed to recognize ovalbumin resies 257-264 in the context of H2Kb and used to study the role of peptides in positive selection and the response of CD8+ T cells to antigen. Like most TCR transgenics, these mice are somewhat immunodeficient.
